Immunomodulatory mechanisms: Parietaria judaica allergens and Ciona intestinalis bioactive peptides as a models.
iTreg and NK regulatory cells: isolation by magneting cell sorting, gene expression and Flow cytometric analysis.
Study of phenotypic and functional markers of activated Regulatory cells. Cross-talk between regulatory pathways in different Antigen specific induced systems. Strategies for Rare cells detection : isolation, expansion, gene expression and Flow cytometric analysis.
Antigen-specific cytokine responses of innate and adaptive human immune cells
Strumenti / Tecnologie
Human immune cell purification and culture, Molecular biology technologies, Magneting cell sorting, FACS