Chromatin and Nuclear Architecture
Istituto di Tecnologie Biomediche (ITB) and Istituto Nazionale Genetica Molecolare (INGM)
Via Francesco Sforza 35, Milano
Membri del Laboratorio
Federica Lucini, PhD student
Giulia La Macchia, undergraduate student
My group is devoted in understanding how the genome folding occur in the nuclear space finding the right orientation and nuclear position and how this conformation is then maintained or regulated in dynamic physiological processes in health and in disease.
Studying human primary CD4+ T lymphocytes we found an unconventional genomic organization where the heterochromatin is susceptible to DNAse digestion while the euchromatin is insoluble. This CD4+ T cell-specific chromatin conformation clearly distinguish lymphocytes from any other cell population analysed, opening unprecedented perspectives on the relation between chromatin organization and function.
Genome wide analysis with a new technology developed in our laboratory:
the SAMMYseq: https://www.biorxiv.org/content/10.1101/799668v1
Skills in epigenetics and chromatin higher order structures